Permeability Of Hydrophilic :: essays research papers

Permeability of Hydrophilic Supervisors: Vladan Milovic Professor Per Artursson SUMMARY Investigations of the integrity and transport characteristics of 2/4/A1 cells have been done in this report. The cell line was isolated from rat fetal intestinal epithelial cells and transfected with thermolabile SV40 large T antigen. These cells proliferated at 33 Â °C, but eliminated the antigen and ceased proliferating at a non-permissive temperature (39Â °C). At 39Â °C 2/4/A1 cells started to differentiate but simultaneously the cells also underwent massive cell death. When cultured at 37Â °C these cells formed confluent and tight monolayers that seemed to have paracellular transport characteristics similar to that of the human intestine. Transmission electron microscopy confirmed the development of multilayers at 33Â °C, monolayers at 37Â °C and defects in the cell layer due to apoptosis at 39Â °C. Different immunostainings of ZO-1, E-cadherin and vinculin confirmed formation of tight and adherence junctions. Transepithelial resistance reached a plateau of 25-35 Ohm.cm2, which was similar to the small intestine. In transport studies 2/4/A1 cell line monolayers selectively restricted the permeation of hydrophilic permeability markers proportional to molecular weight and discriminated more accurately between the molecules of intermediate molecular weight compared to Caco-2 cells. These results indicated that 2/4/A1 cells could be used as a model for hydrophilic drug absorption. INTRODUCTION The small intestine plays a crucial role in the absorption of drugs and nutrients. Exogenous substances cross a series of barriers during the process of intestinal absorption: (1) the aqueous boundary/mucus layer, (2) a single layer of epithelial cells, and (3) the lamina propria, which contains the blood and lymph vessels that then transport the absorbed drugs to other parts of the body (Artursson 1991). The cell monolayer is comprised of two parallel barriers: the cell membrane and the tight junctions. Most drugs are absorbed by a passive diffusion across the cell membrane by the transcellular route, or across the tight junctions between the cells - the paracellular route. Drug transport can also be carrier mediated, when the drug utilizes transporters located in the cellular membrane. Transcytosis is another kind of active transport, in which macromolecules can be transported across the intestinal epithelial cell in endocytosed vesicles. The hydrophilic and charged drugs are absorbed after passing through the paracellular route, the water-filled channels between the cells (Artursson 1991). Rates and extent of the paracellular transport are, therefore, highly influenced by the structure and size of the tight junctions as well as by the size of the molecules. Only small and hydrophilic drugs can pass between the cells rapidly and completely; permeation of larger molecules can be limited proportionally to their size and lipophilicity (Hillgren et al. 1995). Simple assay methods are needed for drug absorption studies.

Bbmdoc.233

CHRIST UNIVERSITY Hosur Road, Bangalore Department of Management Studies Course Plan-2012-2013 Subject/Code: Organizational Behaviour/BBM 232 |Name of the faculty | Email | |Dr. Jain Mathew |[email  protected] in | |Mr. John Paul |john. [email  protected] in | |Ms.Mary Thomas |mary. [email  protected] in | |Ms. Vinita Seshadri |vinita. [email  protected] in | INTRODUCTION To provide students with thorough knowledge in theory and concepts of organizational behavior, also to equip them to address the contemporary changes related to the behavior and performance of people in organizations today.Teaching and learning approach Each week's teaching sessions will comprise: Four- Hour lecture including student centered activity During which you will be introduced to the topic for that week. It is important to note that the coverage of each topic during the lecture will be incomplete unless you read the references provided and attempt the tutorial questions that cover that topic. It is important to note that for each topic a set of objectives is given and it is your responsibility to ensure that you meet those objectives.Recommended text books Stephen Robbins, Timothy A. Judge, Seema Sanghi, Organizational Behavior, 13th Edition Pearson Education, Journals Human Capital MBA Review HRM Review ICFAI HR HBR Review Case Folio Lecture Schedule: 1. MODULE – Introduction to Organizational Behaviour 1. 1 Objectives †¢ Understand the importance of interpersonal skills in the workplace. †¢ Describe the manager’s function, role and skills. – Week |Topics |Hour |Methodology | | |Nature, importance and purpose of organizations |1 |Discussion | | | | | | |Nov 5 – Nov 10 | | | | | |Managerial Skills by Robert Katz |2 |Lecture (C/PPT) | | |Management Roles by Mintzberg |3 |PPT; video showing various roles | | |Effective versus successful Managerial Activities|4 |Group discussion and reflections | | |- | | | | |Luthan’s study | | | 1B. 2 Objectives: On completion of the material you would be able to: †¢ Define Organization Behaviour †¢ Trace the historical roots of organization behaviour †¢ Explain OB Model †¢ Identify the major behavioral science disciplines that contribute to OB †¢ Identify the challenges and opportunities managers have in applying OB Concepts Week |Topics |Hour |Methodology | | |Introduction to Organization Behaviour – OB Model|1 |Lecture (C/PPT) | | | | | | |Nov 12 –Nov 19 | | | | | |Challenges in handling OB |2 |Group discussion and reflections | | |Contribution from other disciplines |3 |Concept Mapping | | |Case Study |4 |Use as a tool to explain how to approach OB case | | | | |studies | 1+. 2 Reference Prerequisite readings – Chapter 1 Stephen Robbins, Timothy A. Judge , Seema Sanghi , Organizational Behavior, 14th Edition Pearson Education 2. MODULE – II Personality 2. 1 Objectives: On completion of the material you would be able to: †¢ Define Personality, describe how it is measured and factors that determine n individual’s personality †¢ Identify Personality Traits relevant to OB and it’s applications in the workplace |Week |Topics |Hour |Methodology | | |Meaning & Determinants of personality |1 |Concept Mapping | | | | | | | | | | | | | | | | | | | | | |Nov 20 – Dec 4 | | | | | |Theories of personality: Type and Trait theory |2 |Activity: ‘Favourite Personality’ | | |The ‘Big Five’ Personality Traits |3 |Lecture (C/PPT) | | |Experiential Exercise: Big Five Personality |4 |Questionnaire | | |Trait | | | | |Myers-Briggs Indicator |5 |Lecture (C/PPT) | | |Psychoanalytical theory |6 |Lecture (C/PPT) | | |Erickson’s personality |7 |Lecture (C/PPT); Handout | | |Group Activity9-+ |8 |Write up on various personality +-+traits of famous | | | | |personalities | | |Case Study |9 |Case discussion/Written Analysis | 2. 2 Reference: Prerequisi te readings –Ch. 4 Stephen Robbins, Timothy A. Judge , Seema Sanghi , Organizational Behavior, 14th Edition Pearson Education 3. MODULE- III Learning Meaning of learning –– Learning theory of Organisational Behaviour-Classical- Operant conditioning- Cognitive- Observation Theory 3. 1 Objectives: On completion of the material you would be able to: †¢ Define and understand what is Learning †¢ Four theories which explains how learning takes place †¢ Learning principles which needs to be observed in any training programme †¢ Its impact on individual behaviour and organization Week |Topics |Hour |Methodology | | |Meaning of learning |1 |Concept Mapping | | | | | | | | | | | |Dec 5 – Dec 21 | | | | | |Learning process |2 |Discussion | | |Classical conditioning |3 |Lecture (C/PPT) & video | | |Operant conditioning |4 |Lecture (C/PPT) & video | | |Cognitive Theory |5 |Lecture (C/PPT) | | |Social Learning Theory |6 |Group discussion – ‘Role Models’ | | |Group Activity |7 |Presentation on videos depicting various learning | | | | |styles/skits | | |Principles of Learning-Reinforcement and |8 |Lecture (C/PPT) | |Punishment | | | | |Learning Curve |9 |Discussion | | |Case Study |10 |Case discussion/Written Analysis | 3. 2 Reference: Prerequisite readings – Chapter 9 K. Aswathappa, 9th edition, OB-Himalaya Publishing House / Chapter 4- Kavitha Singh-OB Text and Cases-Pearson Education / Chapter 4-OB- Hellriegel & Slocum- Thomson 4. MODULE- IV Attitudes Meaning, Characteristics and functions- Components-the ABC Model- Formation of attitude Meaning, Charecteristics, Components and Formation of attitudes. Change in attitude- Barriers to change- How to minimize the barriers. 4. 1 Objectives On completion of the material you would be able to: †¢ Contrast the three components of an attitude †¢ Discuss attitude and attitude formation †¢ Relate attitude to behaviour Week |Topics |Hour |Me thodology | | |Meaning, Characteristics and functions |1 |Concept Mapping | | | | | | | | | | | |Jan 2 – Jan 12 | | | | | |Components-the ABC Model |2 |Lecture (C/PPT) | | |Cognitive Dissonance; Job |3 |GroupDiscussion | | |Satisfaction/Dissatisfaction | | | | |Formation of attitudes- Changing attitudes |4 |Lecture (C/PPT) | | |Barriers to change, How to minimize the barriers |5 |Video | | |Case Study |6 |Case discussion/Written Analysis | 4. 2 Reference: Prerequisite readings – Ch 3 Stephen Robbins, Timothy A. Judge , Seema Sanghi , Organizational Behavior, 14th Edition Pearson Education 5. MODULE – V – PERCEPTION Meaning and definition – Need, Factors influencing perception, perceptual consistency, Context and definition,. 5. 1 Objectives: On completion of the material you would be able to: †¢ Understand importance and factors contributing for perception †¢ Understand the concepts in perceptual Organization Understand factors contri buting towards the interpretation of data |Week |Topics |Hour |Methodology | | |Meaning, nature and importance of Perception |1 |Concept Mapping | | | | | | | | | | | |Jan 21 – Jan 31| | | | | |Factors influencing perception |2 |Lecture (C/PPT) | | |Experiential Exercise 1 |3 |Story telling based on pictures | | |Perceptual Process |4 |Group discussion | | |Interpersonal perception 5 |Video | | |Case Study |6 |Case discussion/Written Analysis | 5. 2 Reference Prerequisite readings – Chapter 6 K. Aswathappa, 9th edition, OB-Himalaya Publishing House 6. MODULE –VI Group Behaviour and Group Dynamics 6. 1 Objectives: On completion of the module you would be able to: †¢ Analyse the different stages of group formation and development. †¢ Differentiate formal and informal groups. †¢ Know the factors that increase or decrease group cohesiveness †¢ Differentiate between groups and teams. †¢ Discuss the conditions for building successful teams. Week |Topics |Hour |Methodology | | |Meaning and types of groups |1 |Concept Mapping | | | | | | | | | | | |Feb 1 – Feb 12 | | | | | |Formation of groups |2 |Activity + Reflection | | |Characteristics of Groups (Size, Norms, Status, |3 |Lecture (C/PPT) | | |Roles) | | | | |Characteristics of Groups (Cohesiveness, |4 |Lecture (C/PPT) | | |Groupthink, Groupshift) | | | | |Group decision making |5 |Lecture (C/PPT) | | |Groups vs Teams |6 |Discussion | 6. 2 Reference: *Aswathappa –Ch. 14 and 15, Organizational Behavior (Text, cases and Games), 9th Edition, Himalaya Publication 7. MODULE – VII Leadership 7. 1 Objectives: On completion of this module you would be able to: †¢ Differentiate Leadership and management. †¢ Leadership styles and their impact on followers. †¢ Transformational leadership, women as leaders and charismatic leadership are emerging issues in leadership. Week |Topics |Hour |Methodology | | |Meaning, concept and Differences wit h managers |1 |Concept Mapping | | | | | | | | | | | |Feb 13 – Feb 23| | | | | |Leadership Styles |2 |Lecture (C/PPT) | | |Managerial Grid; Situational Leadership (Hersey &|3 |Lecture (C/PPT) | | |Blanchard) | | | | |Tannenbaum and Schmidt; Path Goal theory |4 |Lecture (C/PPT) | | |Transactional, Transformational & Charismatic |5 |Presentations by students | | |leaders | | | | |Communication: Meaning and importance, barriers |6 |Discussion | 7. 2 Reference: *Aswathappa –Ch. 18, Organizational Behavior (Text, cases and Games), 9th Edition, Himalaya Publication 8. MODULE – VIII Motivation 8. 1 Objectives: On completion of the material you would be able to: †¢ Identify major content theories of work motivation Explain the major process theories of work motivation †¢ Understand theories of motivation and evaluate their applicability today |Week |Topics |Hour |Methodology | | |Meaning and Concept of motivation |1 |Concept Mapping | | | | | | | | | | | |Feb 25 – Mar 4 | | | | | |Content Theories of Motivation – Two factor |2 |Presentations by students | | |theory, Theory X & Y | | | | |Alderfer ERG Theory; McClleland’s need |3 |Presentations by students | | |classification | | | | |Process Theories- Vroom’s Expectancy Theory, |4 |Lecture (C/PPT) | | |Porter-Lawler theory | | | | |Case Study |5 |Case discussion/Written Analysis | 8. 2 Reference: Prerequisite readings – Chapter 11- K. Aswathappa, 9th edition, OB-Himalaya Publishing House / Chapter 6- Stephen Robbins, Timothy A. Judge , Seema Sanghi / Chapter 8- Fred Luthans- OB- 9th Edition Mc Graw Hill. 9. MODULE-IX- Organizational Change 9. 1 Objectives: On completion of the module you would be able to understand †¢ The nature, level, need and types of change. †¢ The reasons for change in organization. †¢ The reasons why people resist change in organization. †¢ The change process. Week |Topics |Hour |Methodology | | |Nat ure and Levels of Change |1 |Concept Mapping | | | | | | | | | | | |Feb 25 – Mar 4 | | | | | |Types and Forces for Change & Lewin’s Model for |2 |Lecture (C/PPT) | | |Change Management | | | | |Change Process ( Six Stages) |3 |Lecture (C/PPT) | | |Resistance to change (RTC) and methods dealing |4 |Discussions | | |with RTC | | | 9. 2 Reference: *Aswathappa –Ch. 22, Organizational Behavior (Text, cases and Games), 9th Edition, Himalaya Publication Continuous Internal Assessment CIA 1 – Mid Semester Examinations for 2 hours and 50 marks CIA 2 – Comprising of the following 3 components of 10 marks each: i) Article Review – The student will be required to read a specified article and present the review in the following format †¢ Name of the Author, Year of Publication, Title of the article †¢ Objective of the study †¢ Methodology adopted – o Sample (Technique and size) o Tools o Analysis †¢ Findings of the study †¢ Recommendations of the author (ii) MCQ test. (iii) Case analysis – The case must be analyzed in the following manner †¢ Critical analysis o Key facts in the case o Central problem in the case o Theoretical concept it relates to †¢ Answer the Questions †¢ Recommendations/Suggestions + Conclusion CIA 3 – Comprising of the following 3 components of 10 marks each: (i) Written Case analysis of a comprehensive case following the format specified above. (ii) MCQ conducted through LMS (iii) Class participation evaluated through Q, Activities,

Using Qualitative And Qualitative Research - 1069 Words

The authors argue that mixed methods sampling with well-established qualitative and quantitative techniques can answer the research question in health sciences posted by mixed methods research designs in a new way. And data collection procedures for the mixed methods designs should based on concurrent and sequential forms of data collection, in order to make sure that the qualitative results can explain and interpret the findings of a quantitative study, and meanwhile, mixed methods are used to overcome a weakness in using one method with the strengths of another. I Main Points: There are three main points from chapter seven, which are 1) sampling approaches used in quantitative and qualitative research; 2) types of sampling in mixed†¦show more content†¦Probability sampling techniques are most likely to attain from record participants’ attitude and behavior which can record as statistics, and investigators analyze the data to get the result. 2) types of sampling in mixed methods study Sampling plan for mixed methods study can be classified into three types, which are 1) interdependent; 2) independent and 3) combined. Interdependent mixed methods sampling frames usually use to for a particular project through the joint use of probability and purposeful techniques. Independent mixed methods sampling frames are proposing at least two distinct samples, therefore, a primary purposeful sampling technique is chosen for the qualitative component, and a primary probability sampling technique is chosen for the quantitative component. And the advantage of using an independent sampling plan is to protect against generalizability critiques. Combined mixed methods sampling frames are usually used among published mixed methods studies, researchers will use an independent probability approach to determine the sample for the quantitative component and an interdependent approach to determine a subsample of the participants from the quanti tative component for a subsequent qualitative